BspFN I

3,750.00 ₽ – 15,000.00 ₽

  • CNY: 304.95 ¥ - 1,219.80 ¥

Restriction endonuclease BspFN I

  • Recognition site: CG↑CG / GC↓GC
  • Источник: Bacillus species FN
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E557 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E557S500 uRegular5 000 u/ml3,750.00 ₽
  • CNY: 304.95 ¥
E558L2500 uRegular5 000 u/ml15,000.00 ₽
  • CNY: 1,219.80 ¥

Description

Recognition site and hydrolysis position:

CGCG CG↑CG
GCGC GC↓GC

Source: Bacillus species FN
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
50 75 75 50 100 100

Storage conditions: 20 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 200 μg/ml BSA, 7 mM 2-mercaptoethanol and 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CG methylation
Notes:
References:

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