Description
Recognition site and hydrolysis position:
| CGCG | CG↑CG |
| GCGC | GC↓GC |
Source: Bacillus species FN
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 50 | 75 | 75 | 50 | 100 | 100 |
Storage conditions: 20 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 200 μg/ml BSA, 7 mM 2-mercaptoethanol and 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CG methylation
Notes:
References:
Related products
-
Acc65 I
2,750.00 ₽ – 11,000.00 ₽Select options This product has multiple variants. The options may be chosen on the product page- CNY: 223.63 ¥ - 894.52 ¥
Acc65 I
[:ru]Эндонуклеаза рестрикции Acc65 I[:en]Restriction endonuclease Acc65 I[:]
2,750.00 ₽ – 11,000.00 ₽- CNY: 223.63 ¥ - 894.52 ¥
-
Acs I
3,450.00 ₽ – 13,800.00 ₽Select options This product has multiple variants. The options may be chosen on the product page- CNY: 280.55 ¥ - 1,122.22 ¥
Acs I
[:ru]Эндонуклеаза рестрикции Acs I[:en]Restriction endonuclease Acs I[:]
3,450.00 ₽ – 13,800.00 ₽- CNY: 280.55 ¥ - 1,122.22 ¥
-
Aat II
4,400.00 ₽ – 17,600.00 ₽Select options This product has multiple variants. The options may be chosen on the product page- CNY: 357.81 ¥ - 1,431.23 ¥
Aat II
[:ru]Эндонуклеаза рестрикции Aat II
Сайт узнавания: GACGT↑C / C↓TGCAG[:en]Restriction endonuclease Aat II[:]4,400.00 ₽ – 17,600.00 ₽- CNY: 357.81 ¥ - 1,431.23 ¥