BstMC I

3,000.00 ₽ – 12,000.00 ₽

  • CNY: 243.96 ¥ - 975.84 ¥

Restriction endonuclease BstMC I

  • Recognition site: CGRY↑CG / GC↓YRGC
  • Источник: Bacillus stearothermophilus MC
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 50
  • Температура инактивации, 20 мин при: 80
SKU: SE-E071 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E071S500 uRegular5 000 u/ml3,000.00 ₽
  • CNY: 243.96 ¥
E072L2500 uRegular5 000 u/ml12,000.00 ₽
  • CNY: 975.84 ¥

Description

Recognition site and hydrolysis position:

CGRYCG CGRY↑CG
GCYRGC GC↓YRGC

Source: Bacillus stearothermophilus MC
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B + BSA
Enzyme activity (%):

B G O W Y ROSE
100 75 10 10 50 100

Storage conditions: 10 mM Tris-HCl(pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C. Reagents Supplied with Enzyme 10 X SE-buffer B, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml
Do not use BSA for long incubation.
References:
Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).

Related products