BstSF I

4,370.00 ₽ – 17,480.00 ₽

  • CNY: 355.37 ¥ - 1,421.47 ¥

Restriction endonuclease BstSF I

  • Recognition site: C↑TRYAG / GAYRT↓C
  • Источник: Bacillus stearothermophilus SF
  • Оптимальный буфер: SE-буфер O
  • Оптимальная температура: 60
  • Температура инактивации, 20 мин при: N
SKU: SE-E197 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E197S200 uRegular2 000 - 5 000 u/ml4,370.00 ₽
  • CNY: 355.37 ¥
E198L1000 uRegular2 000 - 5 000 u/ml17,480.00 ₽
  • CNY: 1,421.47 ¥

Description

Recognition site and hydrolysis position:

CTRYAG C↑TRYAG
GAYRTC GAYRT↓C

Source: Bacillus stearothermophilus SF
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O + BSA
Enzyme activity (%):

B G O W Y ROSE
75 25 100 50 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C. Reagents Supplied with Enzyme 10 X SE-buffer O, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).

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