BstSL I

6,000.00 ₽ – 24,000.00 ₽

  • CNY: 487.92 ¥ - 1,951.68 ¥

Restriction endonuclease BstSL I

  • Recognition site: GKGCM↑C / C↓MCGKG
  • Источник: Bacillus stearothermophilus S
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 65
SKU: SE-E561 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E561S500 uRegular10 000 u/ml6,000.00 ₽
  • CNY: 487.92 ¥
E562L2500 uRegular10 000 u/ml24,000.00 ₽
  • CNY: 1,951.68 ¥

Description

Recognition site and hydrolysis position:

GKGCMC GKGCM↑C
CMCGKG C↓MCGKG

Source: Bacillus stearothermophilus S
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
50 100 50 75 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these, 95% can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer G, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by overlapping dcm-methylation (CmCWGG):GKGCCCWGG
Blocked by GKG(5mC)MC methylation
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:

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