BstSN I

3,000.00 ₽ – 12,000.00 ₽

  • CNY: 243.96 ¥ - 975.84 ¥

Restriction endonuclease BstSN I

  • Recognition site: TAC↑GTA / ATG↓CAT
  • Источник: Bacillus stearothermophilus SN
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E065 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E065S200 uRegular5 000 u/ml3,000.00 ₽
  • CNY: 243.96 ¥
E066L1000 uRegular5 000 u/ml12,000.00 ₽
  • CNY: 975.84 ¥

Description

Recognition site and hydrolysis position:

TACGTA TAC↑GTA
ATGCAT ATG↓CAT

Source: Bacillus stearothermophilus SN
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
T7 DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 50 0 10 50 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 100 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at 20°C.
Ligations:
After 5-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration results in star activity
References:
Myakisheva, T.V., Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1995).

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