Description
Recognition site and hydrolysis position:
| TACGTA | TAC↑GTA |
| ATGCAT | ATG↓CAT |
Source: Bacillus stearothermophilus SN
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
T7 DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 100 | 50 | 0 | 10 | 50 | 50 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 100 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at 20°C.
Ligations:
After 5-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration results in star activity
References:
Myakisheva, T.V., Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1995).
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