BstX I

1,100.00 ₽ – 4,400.00 ₽

  • CNY: 89.45 ¥ - 357.81 ¥

Restriction endonuclease BstX I

  • Recognition site: CCANNNNN↑NTGG / GGTN↓NNNNNACC
  • Источник: Bacillus stearothermophilus X
  • Оптимальный буфер: SE-буфер O
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E465 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E465S200 uRegular5 000 u/ml1,100.00 ₽
  • CNY: 89.45 ¥
E466L1000 uRegular5 000 u/ml4,400.00 ₽
  • CNY: 357.81 ¥

Description

Recognition site and hydrolysis position:

CCANNNNNNTGG CCANNNNN↑NTGG
GGTNNNNNNACC GGTN↓NNNNNACC

Source: Bacillus stearothermophilus X
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

B G O W Y ROSE
10 10 100 75 25 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Wise, R., Schildkraut, I. Unpublished observations (1992).

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