Description
5Recognition site and hydrolysis position:
| RGATCY | R↑GATCY |
| YCTAGR | YCTAG↓R |
Source: An E.coli strain that carries the cloned BstX2I gene from Bacillus stearothermophilus X2
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 75 | 100 | 0 | 10 | 25 | 100 |
Storage conditions: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.Reagents Supplied with Enzyme
10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by Dam-methylation (GmATC): RGATCYNotes:
References:
Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).
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