CciN I

2,200.00 ₽ – 8,800.00 ₽

  • CNY: 178.90 ¥ - 715.62 ¥

Restriction endonuclease CciN I

  • Recognition site: GC↑GGCCGC / CGCCGG↓CG
  • Источник: Из штамма E.coli несущего клонированный ген CciN I из Curtobacterium citreus N.
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E203 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E203S500 uRegular10 000 u/ml2,200.00 ₽
  • CNY: 178.90 ¥
E204L2500 uRegular10 000 u/ml8,800.00 ₽
  • CNY: 715.62 ¥
E203TS500 uTurbo10 000 u/ml2,200.00 ₽
  • CNY: 178.90 ¥
E204TL2500 uTurbo10 000 u/ml8,800.00 ₽
  • CNY: 715.62 ¥

Description

Recognition site and hydrolysis position:

GCGGCCGC GC↑GGCCGC
CGCCGGCG CGCCGG↓CG

Source: Curtobacterium citreus N
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Adenovirus-2 DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
25 50 75 75 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of DNA Ad-2 with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y.
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CG methylation.
Notes: High enzyme concentration may result in star activity.
References:
Verchozina, V.A., Degtyarev, S.Kh. Gene 157: 99-100 (1995).

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