Dra I

1,430.00 ₽ – 5,720.00 ₽

  • CNY: 116.29 ¥ - 465.15 ¥

Restriction endonuclease Dra I

  • Recognition site: TTT↑AAA / AAA↓TTT
  • Источник: Deinococcus radiophilus
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E055 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E055TS1000 uTurbo20 000 u/ml1,430.00 ₽
  • CNY: 116.29 ¥
E056TL5000 uTurbo20 000 u/ml5,720.00 ₽
  • CNY: 465.15 ¥
E055S1000 uRegular20 000 u/ml1,430.00 ₽
  • CNY: 116.29 ¥
E056L5000 uRegular20 000 u/ml5,720.00 ₽
  • CNY: 465.15 ¥

Description

Recognition site and hydrolysis position:

TTTAAA TTT↑AAA
AAATTT AAA↓TTT

Source: Deinococcus radiophilus
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
75 100 25 75 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Purvis, I.J., Moseley, B.E.B., Nucleic Acids Res. 11:5467-5474 (1983).

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