DseD I

4,255.00 17,020.00 

  • CNY: 346.02 ¥ - 1,384.07 ¥

Restriction endonuclease DseD I

  • Recognition site: GACNNNN↑NNGTC / CTGNN↓NNNNCAG
  • Источник: Из Deinococcus species D2
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E241 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E241S500 uRegular10 000 u/ml4,255.00 
  • CNY: 346.02 ¥
E242L2500 uRegular10 000 u/ml17,020.00 
  • CNY: 1,384.07 ¥

Description

Recognition site and hydrolysis position:

GACNNNNNNGTC GACNNNN↑NNGTC
CTGNNNNNNCAG CTGNN↓NNNNCAG

Source: Deinococcus species D2
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 75 25 50 100 30

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml
Do not use BSA for long incubation.
References:
Abdurashitov, M.A., Kileva, E.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1996).