Description
Recognition site and hydrolysis position:
| YATR | YA↑TR |
| RTAY | RT↓AY |
Source: Flavobacterium aquatile B15
FaiI cleaves 4 expected recognition sites as well as several other sites with a weaker activity.
In the case of long incubation with FaiI DNA can be digested to small oligos.
Unit definition:
One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 50°C in a total reaction volume of 20 μl.
Assayed on:
Double-stranded oligonucleotide
5`- CGAGTTCA^TAGCTGGGCCCAAC -3`
3`- GCTCAAGT^ATCGACCCGGGTTG -5`
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 100 | 50 | 10 | 25 | 25 | 100 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme about 90% of the pUC19 DNA fragments can be ligated with DNA Ligase and recut.Reagents Supplied with Enzyme
10 X SE-buffer B
Non-specific hydrolisis:
Reagents Supplied with Enzyme:
Methylation sensitivity:
Notes:
References:
Tarasova G.V., Chernukhin V.A., Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease FaiI // In press
