Fai I

Description

Recognition site and hydrolysis position:

Source: Flavobacterium aquatile B15
Unit definition:
One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 50°C in a total reaction volume of 20 μl.
Assayed on:
Double-stranded oligonucleotide
5`- CGAGTTCA^TAGCTGGGCCCAAC -3`
3`- GCTCAAGT^ATCGACCCGGGTTG -5`
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme about 90% of the pUC19 DNA fragments can be ligated with DNA Ligase and recut. Reagents Supplied with Enzyme 10 X SE-buffer B
Non-specific hydrolisis:
Reagents Supplied with Enzyme:
Methylation sensitivity:
Notes:
References:
Tarasova G.V., Chernukhin V.A., Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease FaiI // In press