Fbl I

1,500.00 ₽ – 6,000.00 ₽

  • CNY: 121.98 ¥ - 487.92 ¥

Restriction endonuclease Fbl I

  • Recognition site: GT↑MKAC / CAKM↓TG
  • Источник: Из штамма E.coli несущего клонированный ген Fbl I из Flavobacterium balustinum
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 80
SKU: SE-E271 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E271S100 uRegular1 000-2 000 u/ml1,500.00 ₽
  • CNY: 121.98 ¥
E272L500 uRegular1 000-2 000 u/ml6,000.00 ₽
  • CNY: 487.92 ¥

Description

Recognition site and hydrolysis position:

GTMKAC GT↑MKAC
CAKMTG CAKM↓TG

Source: An E.coli strain that carries the cloned Fbl I gene from Flavobacterium balustinum
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
50 75 0 50 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, and 50% glycerol; Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Degtyarev, S.K. Unpublished observations.

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