Fok I

Fok I

Price range: 1 500 ₽ through 6 000 ₽

Restriction endonuclease Fok I

  • Recognition site:
    5'… GGATG(N)9▼ …3'
    3'… CCTAC(N)13▲ …5'
  • Source: Flavobacterium okeanokoites
  • Optimal buffer: SE-buffer Y
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E247 Category:

SKUРackagePackageVariantConcentrationPrice 
E247S100 uRegular1 000-2 000 u/ml1 500 ₽
E248L500 uRegular1 000-2 000 u/ml6 000 ₽

Description

Recognition site and hydrolysis position:

GGATG(N)9 GGATG(N)9↑
CCTAC(N)13 CCTAC(N)13↓

Source: An E.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
50 50 25 25 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 1 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: Overdigestions of > 5 units of Fok I per 1μg of DNA and incubations > 2 hours are not recommended.
References:
Sugisaki, H., Kanazawa, S., Gene 16: 73-78 (1981).

Additional Information

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