Hga I

Hga I

Price range: 4 700 ₽ through 18 800 ₽

Restriction endonuclease Hga I

  • Recognition site:
    5'… GACGC(N)5▼ …3'
    3'… CTGCG(N)10▲ …5'
  • Source: An E.coli strain that carries the cloned HgaI gene from Haemophilus gallinarum
  • Optimal buffer: SE-buffer B
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E461 Category:

SKUРackagePackageVariantConcentrationPrice 
E461S50 uRegular1 000 u/ml4 700 ₽
E462L250 uRegular1 000 u/ml18 800 ₽

Description

Recognition site and hydrolysis position:

GACGC(N)5 GACGC(N)5↑
CTGCG(N)10 CTGCG(N)10↓

Source: An E.coli strain that carries the cloned HgaI gene from Haemophilus gallinarum
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of DNA pBR322 in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
DNA pBR322
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 75 10 25 50 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
Incubation with > 2 units of HgaI per 1 μg of DNA and digestion > 1 hour are not recommended. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CG methylation.
Notes:
References:
Brown N.L., Smith M. Proc. Natl. Acad. Sci. U. S. A. 74: 3213-3216 (1977).Sugisaki H. Gene 3: 17-28 (1978).

Additional Information

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