Description
Recognition site and hydrolysis position:
| AGGCCT | AGG↑CCT |
| TCCGGA | TCC↓GGA |
Source: Planococcus citreus
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 75 | 75 | 50 | 25 | 100 | 100 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C.Reagents Supplied with Enzyme
10 X SE-buffer Y.
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by overlapping Dcm-methylation (CmCWGG): AGGCCTGG
Notes: At 37°C activity is 50-75% from maximum
References:
Gonchar, D.A., Shinkarenko, N.M., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).
