Description
TaqSE DNA polymerase is complex mix of thermostable DNA polymerase that possesses a 5` -> 3` polymerase activity, 3` -> 5` exonuclease (proofreading) activity and a double strand specific 5` -> 3` exonuclease activity.
It may increase yield of reaction product compare to Taq DNA polymerase.
Application: long high fidelity primer extension reaction.
Source: Isolated from E.coli strain that carries the recombinant plasmids
Unit definition: TaqSE DNA polymerase is complex mix of thermostable DNA polymerase that possesses a 5` -> 3` polymerase activity, 3` -> 5` exonuclease (proofreading) activity and a double strand specific 5` -> 3` exonuclease activity.
It may increase yield of reaction product compare to Taq DNA polymerase.
Application: long high fidelity primer extension reaction.
Reaction buffer: SE-buffer DNA polymerase Taq
Storage conditions: 20 mM Tris-HCl (pH 7.6); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% Triton X-100; 50% glycerol. Store at -20°C.
Quality control: The enzyme is purified free of contaminating endonucleases andexonucleases.