Xba I

Description

Recognition site and hydrolysis position:

Source: An E.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-/HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dam-/HindIII-digest)
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O, BSA (except E141m, E141T and E142T).
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dam-methylation (G^mATC): TCTAGATCNotes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Zain, B,S., Roberts, R.J. J. Mol. Biol. 115: 249-255 (1977).
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from “Medical genetics” V.6, No 8, pp 29-36, 2007