Description
Recognition site and hydrolysis position:
| CCCGGG | C↑CCGGG |
| GGGCCC | GGGCC↓C |
Source: An E.coli strain, that carries the cloned gene XmaI from Xanthomonas malvacearum
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus -2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Adenovirus -2 DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 75 | 50 | 0 | 0 | 100 | 50 |
Storage conditions: 20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37°C
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Endow, S.A., Roberts, R.J. J. Mol. Biol. 112: 521-529 (1977).
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