Xma I

3,630.00 ₽ – 14,520.00 ₽

  • CNY: 282.31 ¥ - 1,129.22 ¥

Restriction endonuclease Xma I

  • Recognition site: C↑CCGGG / GGGCC↓C
  • Источник: Из штамма E.coli несущего клонированный ген Xma I из Xanthomonas malvacearum
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E233 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E233S300 uRegular3 000 u/ml3,630.00 ₽
  • CNY: 282.31 ¥
E234L1500 uRegular3 000 u/ml14,520.00 ₽
  • CNY: 1,129.22 ¥

Description

Recognition site and hydrolysis position:

CCCGGG C↑CCGGG
GGGCCC GGGCC↓C

Source: An E.coli strain, that carries the cloned gene XmaI from Xanthomonas malvacearum
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus -2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Adenovirus -2 DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 50 0 0 100 50

Storage conditions: 20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Endow, S.A., Roberts, R.J. J. Mol. Biol. 112: 521-529 (1977).

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