SibEnzyme restriction enzymes database

Список ферментов

Bpu10 I

Enzyme name Bpu10 I
Prototype Bpu10I
SKU SE-E149
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CC▼TNAGC …3'
3'… GGANT▲CG …5'
Source An E.coli strain, that carries recombinant plasmids
Optimal buffer SE-buffer K
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1025505025100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C
Ligation After 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these 90% can be recut. In the presence of 10% PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 1 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer K
Notes High enzyme concentration may result in star activity or incomplete DNA cleavage. We recommend increasing incubation time instead of using an excess of Bpu10I. The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
References
  1. Degtyarev, S.Kh., Zilkin, P.A., Prihodko, G.G., Repin, V.E., Rechkunova, N.I. Mol. Biol. (Mosk) 23: 1051-1056 (1989). Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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Bpu10 I

Enzyme name Bpu10 I
Prototype Bpu10I
SKU SE-E149
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CC▼TNAGC …3'
3'… GGANT▲CG …5'
Source An E.coli strain, that carries recombinant plasmids
Optimal buffer SE-buffer K
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1025505025100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C
Ligation After 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these 90% can be recut. In the presence of 10% PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 1 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer K
Notes High enzyme concentration may result in star activity or incomplete DNA cleavage. We recommend increasing incubation time instead of using an excess of Bpu10I. The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
References
  1. Degtyarev, S.Kh., Zilkin, P.A., Prihodko, G.G., Repin, V.E., Rechkunova, N.I. Mol. Biol. (Mosk) 23: 1051-1056 (1989). Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006