SibEnzyme restriction enzymes database

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Emi I

Enzyme name Emi I
Prototype Ksp632I
SKU SE-E609
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CTCTTC(N)1▼ …3'
3'… GAGAAG(N)4▲ …5'
Source Exiguobacterium mexicanum 6 Definition of Activity Unit One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Substrate for Activity Determination: λ DNA
Optimal buffer SE buffer Y
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
7550252510025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol Store at -20°C Ligase Compatibility After 5-fold overdigestion with Emi I, 95% of the DNA fragments can be ligated with T4 DNA Ligase and 80% of those can be recut
Ligation After digestion with a 5-fold excess of enzyme, 95% of DNA fragments can be ligated by DNA ligase, and 80% of these can be recut.
Nonspecific hydrolysis A 50 µl reaction containing 1 µg of DNA and 5 units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour. Oligonucleotide Assay No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 5 units of restriction endonuclease for 3 hours. Supplied with the Enzyme 10x SE Buffer Y Enzyme Properties When using a buffer other than the optimal (supplied) SE-Buffer, it may be necessary to add more enzyme to achieve complete digestion. Reference Abdurashitov M.A., Gonchar D.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Nikonova A.A., Degtyarev S.Kh. (2025) The restriction endonuclease EmiI, an isoschizomer of Ksp632I, recognizes the non-palindromic DNA sequence 5′-CTCTTC(1/4)-3′. DNA-Processing Enzymes, 2025, Vol. 4.
Methylation sensitivity
Supplied with enzyme 10 X SE-buffer Y.
Notes
References
  1. Abdurashitov M.A., Gonchar D.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Nikonova A.A., Degtyarev S.Kh. The restriction endonuclease EmiI, an isoschizomer of Ksp632I, recognizes the non-palindromic DNA sequence 5′-CTCTTC(1/4)-3′. // DNA-Processing Enzymes, 2025(4)

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Emi I

Enzyme name Emi I
Prototype Ksp632I
SKU SE-E609
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CTCTTC(N)1▼ …3'
3'… GAGAAG(N)4▲ …5'
Source Exiguobacterium mexicanum 6 Definition of Activity Unit One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Substrate for Activity Determination: λ DNA
Optimal buffer SE buffer Y
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
7550252510025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol Store at -20°C Ligase Compatibility After 5-fold overdigestion with Emi I, 95% of the DNA fragments can be ligated with T4 DNA Ligase and 80% of those can be recut
Ligation After digestion with a 5-fold excess of enzyme, 95% of DNA fragments can be ligated by DNA ligase, and 80% of these can be recut.
Nonspecific hydrolysis A 50 µl reaction containing 1 µg of DNA and 5 units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour. Oligonucleotide Assay No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 5 units of restriction endonuclease for 3 hours. Supplied with the Enzyme 10x SE Buffer Y Enzyme Properties When using a buffer other than the optimal (supplied) SE-Buffer, it may be necessary to add more enzyme to achieve complete digestion. Reference Abdurashitov M.A., Gonchar D.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Nikonova A.A., Degtyarev S.Kh. (2025) The restriction endonuclease EmiI, an isoschizomer of Ksp632I, recognizes the non-palindromic DNA sequence 5′-CTCTTC(1/4)-3′. DNA-Processing Enzymes, 2025, Vol. 4.
Methylation sensitivity
Supplied with enzyme 10 X SE-buffer Y.
Notes
References
  1. Abdurashitov M.A., Gonchar D.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Nikonova A.A., Degtyarev S.Kh. The restriction endonuclease EmiI, an isoschizomer of Ksp632I, recognizes the non-palindromic DNA sequence 5′-CTCTTC(1/4)-3′. // DNA-Processing Enzymes, 2025(4)