SibEnzyme restriction enzymes database

Список ферментов

Fae I

Enzyme name Fae I
Prototype NlaIII
SKU SE-E495
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CATG▼ …3'
3'… ▲GTAC …5'
Source Flavobacterium aquatile N3
Optimal buffer SE-buffer FaeI
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550101075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on pUC19 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more then 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of pUC19 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by C m ATG methylation
Supplied with enzyme 10 X SE-buffer FaeI, BSA
Notes To obtain 100% activity, BSA should be added the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S.. Unpublished observations (2006)

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Fae I

Enzyme name Fae I
Prototype NlaIII
SKU SE-E495
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CATG▼ …3'
3'… ▲GTAC …5'
Source Flavobacterium aquatile N3
Optimal buffer SE-buffer FaeI
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550101075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on pUC19 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more then 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of pUC19 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by C m ATG methylation
Supplied with enzyme 10 X SE-buffer FaeI, BSA
Notes To obtain 100% activity, BSA should be added the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S.. Unpublished observations (2006)