SibEnzyme restriction enzymes database

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Hinf I

Enzyme name Hinf I
Prototype HinfI
SKU SE-E075
Turbo version Available
High-concentration version Available
Recognition site
5'… G▼ANTC …3'
3'… CTNA▲G …5'
Source An E.coli strain, that carries the cloned gene HinfI from Haemophilus influenzae
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
25751007575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O.
Notes
References
  1. Hutchison, C.A., Barrell, B.G. Unpublished observations. (1992) Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007

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Hinf I

Enzyme name Hinf I
Prototype HinfI
SKU SE-E075
Turbo version Available
High-concentration version Available
Recognition site
5'… G▼ANTC …3'
3'… CTNA▲G …5'
Source An E.coli strain, that carries the cloned gene HinfI from Haemophilus influenzae
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
25751007575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O.
Notes
References
  1. Hutchison, C.A., Barrell, B.G. Unpublished observations. (1992) Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007