SibEnzyme restriction enzymes database

Список ферментов

HspA I

Enzyme name HspA I
Prototype HhaI
SKU SE-E069
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼CGC …3'
3'… CGC▲G …5'
Source An E.coli strain that carries the cloned HspA I gene from Haemophilus species A1
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
50502525100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation 5'-G(5mC)GC-3'/3'-CG(5mC)G-5'. Not blocked by methylation 5'-GCG(5mC)-3'/3'-CGCG-5'. Cut hemimethylated site:5'- G(5mC)GC-3'/3'-CGCG-5`
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Rechkunova, N.I., Prikhod'ko, E.A., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1994). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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HspA I

Enzyme name HspA I
Prototype HhaI
SKU SE-E069
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼CGC …3'
3'… CGC▲G …5'
Source An E.coli strain that carries the cloned HspA I gene from Haemophilus species A1
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
50502525100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation 5'-G(5mC)GC-3'/3'-CG(5mC)G-5'. Not blocked by methylation 5'-GCG(5mC)-3'/3'-CGCG-5'. Cut hemimethylated site:5'- G(5mC)GC-3'/3'-CGCG-5`
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Rechkunova, N.I., Prikhod'ko, E.A., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1994). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006