SibEnzyme restriction enzymes database

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KroN I

Enzyme name KroN I
Prototype NaeI
SKU SE-E599
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GCC▼GGC …3'
3'… CGG▲CCG …5'
Source Kocurea rosea56
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1007510257550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pSXH7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Plasmid DNA pSXH7 (13,092 bp). pSXH7 was constructed by ligation of the pUC19/BamHI vector with a BstXI fragment of genomic DNA from Sporosarcina species 9 (GC content 70–76%). The plasmid contains 42 recognition sites for the restriction endonuclease KroNI.
Storage conditions 20 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 100 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C; Shelf life: 2 years.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis The pattern of specific DNA cleavage is unchanged after incubation of 1 μg of DNA with 4 units of the enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CpG methylation at the sequence 5′-GC(5mC)GGC-3′ / 3′-CGG(5mC)CG-5′.
Supplied with enzyme
Notes After incubation with 2 units of the enzyme for 3 hours, no nonspecific cleavage of single- or double-stranded oligonucleotides is observed. Efficient DNA hydrolysis by the enzyme requires two or more recognition sites.

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KroN I

Enzyme name KroN I
Prototype NaeI
SKU SE-E599
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GCC▼GGC …3'
3'… CGG▲CCG …5'
Source Kocurea rosea56
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1007510257550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pSXH7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Plasmid DNA pSXH7 (13,092 bp). pSXH7 was constructed by ligation of the pUC19/BamHI vector with a BstXI fragment of genomic DNA from Sporosarcina species 9 (GC content 70–76%). The plasmid contains 42 recognition sites for the restriction endonuclease KroNI.
Storage conditions 20 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 100 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C; Shelf life: 2 years.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis The pattern of specific DNA cleavage is unchanged after incubation of 1 μg of DNA with 4 units of the enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CpG methylation at the sequence 5′-GC(5mC)GGC-3′ / 3′-CGG(5mC)CG-5′.
Supplied with enzyme
Notes After incubation with 2 units of the enzyme for 3 hours, no nonspecific cleavage of single- or double-stranded oligonucleotides is observed. Efficient DNA hydrolysis by the enzyme requires two or more recognition sites.