SibEnzyme restriction enzymes database

Список ферментов

Kzo9 I

Enzyme name Kzo9 I
Prototype MboI
SKU SE-E187
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼GATC …3'
3'… CTAG▲ …5'
Source Kurthia zopfii 9
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50100505050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by overlapping Dam methylation (G m ATC) GATC. Blocked by overlapping CG methylation C G AT m C G. Cleaved of DNA is impaired by overlapping CG methylation GAT m C G.
Supplied with enzyme 10 X SE-buffer G
Notes
References
  1. Degtyarev, S.Kh., Rechkunova, N.I., Grinev, A.A., Dedkov, V,S., Izv.Sib.Otd.Akad.Nauk SSSR 15: 25-26 (1989). M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 29-38, 2006 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006 Kzo9I

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Kzo9 I

Enzyme name Kzo9 I
Prototype MboI
SKU SE-E187
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼GATC …3'
3'… CTAG▲ …5'
Source Kurthia zopfii 9
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50100505050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by overlapping Dam methylation (G m ATC) GATC. Blocked by overlapping CG methylation C G AT m C G. Cleaved of DNA is impaired by overlapping CG methylation GAT m C G.
Supplied with enzyme 10 X SE-buffer G
Notes
References
  1. Degtyarev, S.Kh., Rechkunova, N.I., Grinev, A.A., Dedkov, V,S., Izv.Sib.Otd.Akad.Nauk SSSR 15: 25-26 (1989). M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 29-38, 2006 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006 Kzo9I