SibEnzyme restriction enzymes database

Список ферментов

Rsa I

Enzyme name Rsa I
Prototype RsaI
SKU SE-E113
Turbo version Available
High-concentration version Not available
Recognition site
5'… GT▼AC …3'
3'… CA▲TG …5'
Source Rhodopseudomonas sphaeroides
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
100500507550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes
References
  1. Lynn, S.P., Cohen, L.K., Kaplan, S., Gardner, J.F. J.Bacteriol. 142: 380-383 (1980). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

Поиск по названию

Rsa I

Enzyme name Rsa I
Prototype RsaI
SKU SE-E113
Turbo version Available
High-concentration version Not available
Recognition site
5'… GT▼AC …3'
3'… CA▲TG …5'
Source Rhodopseudomonas sphaeroides
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
100500507550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes
References
  1. Lynn, S.P., Cohen, L.K., Kaplan, S., Gardner, J.F. J.Bacteriol. 142: 380-383 (1980). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006