SibEnzyme restriction enzymes database

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Xba I

Enzyme name Xba I
Prototype XbaI
SKU SE-E141
Turbo version Available
High-concentration version Available
Recognition site
5'… T▼CTAGA …3'
3'… AGATC▲T …5'
Source An E.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575100507525
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-/HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dam-/HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dam-methylation (G m ATC) TCTA GA TC
Supplied with enzyme 10 X SE-buffer O, BSA (except E141m, E141T and E142T).
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Zain, B,S., Roberts, R.J. J. Mol. Biol. 115: 249-255 (1977). Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007

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Xba I

Enzyme name Xba I
Prototype XbaI
SKU SE-E141
Turbo version Available
High-concentration version Available
Recognition site
5'… T▼CTAGA …3'
3'… AGATC▲T …5'
Source An E.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575100507525
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-/HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dam-/HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dam-methylation (G m ATC) TCTA GA TC
Supplied with enzyme 10 X SE-buffer O, BSA (except E141m, E141T and E142T).
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Zain, B,S., Roberts, R.J. J. Mol. Biol. 115: 249-255 (1977). Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007