BseX3 I

Enzyme nameBseX3 I
PrototypeXmaIII
Known analogsBstZI, EagI, EclXI, Eco52I, XmaIII
SKUSE-E263
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… C▼GGCCG …3'
3'… GCCGG▲C …5'
SourceBacillus stearothermophilus X3
Optimal bufferSE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature50 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
1025100501025
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 8.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. Storage at -70 °C is recommended for periods longer than 3 months
LigationAfter 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated. Of these 80% can be recut
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivityBlocked by CG methylation.
Reagents supplied with enzyme10 X SE-buffer O
References
  1. Unpublished data Kileva, E.V., Dedkov, V.S., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.K Unpublished observations (1996).
// Код для некролога