Sbf I

Enzyme nameSbf I
PrototypeSse8387I
Known analogsSdaI, Sse8387I
SKUSE-E101
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CCTGCA▼GG …3'
3'… GG▲ACGTCC …5'
SourceAn E.coli strain that carries the cloned Sbf I gene from Streptomyces species Bf61
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
75500010050
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these more than 90% canrecut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
NotesHigh enzyme concentration may result in star activity.
References
  1. Unpublished data Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.Kh Unpublished observations (1994).
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