SibEnzyme restriction enzymes database

Список ферментов

Bmo I

Enzyme name Bmo I
Prototype SanDI
SKU SE-E607
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GG▼GWCCC …3'
3'… CCCWG▲GG …5'
Source Brevundimonas mongoliensis 53 Definition of Activity Unit One unit is defined as the amount of enzyme required to digest 1 µg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Substrate for Activity Determination T7 DNA
Optimal buffer SE buffer W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550251005075
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol Store at -20°C Ligase Compatibility After 3-fold overdigestion with Bmo I, >90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Ligation After digestion with a 3-fold excess of enzyme, more than 90% of DNA fragments can be ligated by DNA ligase and can be recut.
Nonspecific hydrolysis A 50 µl reaction containing 1 µg of DNA and 4 units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour Oligonucleotide Assay No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 2 units of restriction endonuclease for 3 hours.
Methylation sensitivity Blocked by overlapping CG-methylation GGGWCC(5mC)G Supplied with the Enzyme 10x SE Buffer W Enzyme Properties When using a buffer other than the optimal (supplied) SE-Buffer, it may be necessary to add more enzyme to achieve complete digestion.
Supplied with enzyme 10 X SE-buffer W.
Notes
References
  1. Gonchar D.A., Abdurashitov M.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Ryzhova V.E., Degtyarev S.Kh. (2025) The restriction endonuclease BmoI from the bacterial strain Brevundimonas mongoliensis 53 recognizes the DNA sequence 5′-GG^GWCCC-3′. DNA-Processing Enzymes, 2025(2). DOI: 10.26213/3034-4301.2025.5.2.002

Поиск по названию

Bmo I

Enzyme name Bmo I
Prototype SanDI
SKU SE-E607
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GG▼GWCCC …3'
3'… CCCWG▲GG …5'
Source Brevundimonas mongoliensis 53 Definition of Activity Unit One unit is defined as the amount of enzyme required to digest 1 µg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Substrate for Activity Determination T7 DNA
Optimal buffer SE buffer W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550251005075
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol Store at -20°C Ligase Compatibility After 3-fold overdigestion with Bmo I, >90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Ligation After digestion with a 3-fold excess of enzyme, more than 90% of DNA fragments can be ligated by DNA ligase and can be recut.
Nonspecific hydrolysis A 50 µl reaction containing 1 µg of DNA and 4 units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour Oligonucleotide Assay No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 2 units of restriction endonuclease for 3 hours.
Methylation sensitivity Blocked by overlapping CG-methylation GGGWCC(5mC)G Supplied with the Enzyme 10x SE Buffer W Enzyme Properties When using a buffer other than the optimal (supplied) SE-Buffer, it may be necessary to add more enzyme to achieve complete digestion.
Supplied with enzyme 10 X SE-buffer W.
Notes
References
  1. Gonchar D.A., Abdurashitov M.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Ryzhova V.E., Degtyarev S.Kh. (2025) The restriction endonuclease BmoI from the bacterial strain Brevundimonas mongoliensis 53 recognizes the DNA sequence 5′-GG^GWCCC-3′. DNA-Processing Enzymes, 2025(2). DOI: 10.26213/3034-4301.2025.5.2.002