Alu I

1,010.00 4,040.00 

  • CNY: 14.32 ¥ - 57.28 ¥

Restriction endonuclease Alu I

  • Recognition site: AG↑CT / TC↓GA
  • Источник: Из штамма E.coli несущего клонированный ген Alu I изArthrobacter luteus
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: E015 Category:

Description

Recognition site:

AG↑CT
TC↓GA

Source: An. E.coli strain that carries the cloned Alu I gene from Arthrobacter luteus
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 75 10 50 100 50

Storage conditions: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 200 μg/ml BSA; 1 mM DTT, 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 4 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Roberts, R.J., Myers, P.A., Morrison, A., Murray, K. J. Mol.Biol. 102: 157-165 (1976).
Degtyarev S.Kh., Kolyhalov A.A., Rechkunova N.I., Dedkov V.S., Zhilkin P.A. BsiI – novel unusual restriction endonuclease.// Molecular Biology(Moscow), Vol.24, No. 1, 244-247 (1990). (In Russian)
Repin V.E., Polshina S.V., Bozhko N.A., Degtyarev S.Kh. The cultivation of Bacillus species 21 – Bse21I restriction endonuclease producer.// Izvestia SB SA USSR, Biology sciences seria, Molecular byology, vol.1, 108-111 (1989) (In Russian)
Dedkov V.S., Degtyarev S.Kh., Rechkunova N.I., Serov G.D., Puchkova L.I., Vaitkiavichus D.P., Kyudulene E.-L.Yu., Janulaitis E.-A.A. Staphilococcus saprophyticus – a strain producer of restriction endonuclease SsrI.// Soviet Union patent SU 1486512 (1989). (In Russian)
Degtyarev S.Kh., Repin V.E., Rechkunova N.I., Shevchenko L.S., Ivanova E.P., Rasskazov V.A. Bacillus species 21 – a strain producer of restriction endonuclease Bse21I.// Soviet Union patent SU 1518372 (1989). (In Russian)

Available Options

SKUРackage sizePackageVariantConcentrationPrice 
E015S200 uRegular2 000 - 5 000 u/ml1,010.00 
E016L1000 uRegular2 000 - 5 000 u/ml4,040.00 
E015TS200 uTurbo2 000 - 5 000 u/ml1,010.00 
E016TL1000 uTurbo2 000 - 5 000 u/ml4,040.00