Bmt I

5,500.00 ₽ – 22,000.00 ₽

  • CNY: 425.21 ¥ - 1,700.82 ¥

Restriction endonuclease Bmt I

  • Recognition site: GCTAG↑C / C↓GATCG
  • Источник: Из штамма E.coli несущего клонированный ген Bmt I из Bacillus megaterium S2
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E457 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E457TS1000 uTurbo20 000 u/ml5,500.00 ₽
  • CNY: 425.21 ¥
E458TL5000 uTurbo20 000 u/ml22,000.00 ₽
  • CNY: 1,700.82 ¥
E457S1000 uRegular20 000 u/ml5,500.00 ₽
  • CNY: 425.21 ¥
E458L5000 uRegular20 000 u/ml22,000.00 ₽
  • CNY: 1,700.82 ¥

Description

Recognition site and hydrolysis position:

GCTAGC GCTAG↑C
CGATCG C↓GATCG

Source: An E.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (Hind III-digest)
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
10 50 50 100 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13.BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.
References:
Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K. Biotekhnologiya 1: 11-15 (2003).

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