BseX3 I

3,000.00 ₽ – 12,000.00 ₽

  • CNY: 234.27 ¥ - 937.08 ¥

Restriction endonuclease BseX3 I

  • Recognition site: C↑GGCCG / GCCGG↓C
  • Источник: Bacillus stearothermophilus X3
  • Оптимальный буфер: SE-буфер O
  • Оптимальная температура: 50
  • Температура инактивации, 20 мин при: 80
SKU: SE-E263 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E263S200 uRegular5 000-10 000 u/ml3,000.00 ₽
  • CNY: 234.27 ¥
E264L1000 uRegular5 000-10 000 u/ml12,000.00 ₽
  • CNY: 937.08 ¥

Description

Recognition site and hydrolysis position:

CGGCCG C↑GGCCG
GCCGGC GCCGG↓C

Source: Bacillus stearothermophilus X3
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

B G O W Y ROSE
10 25 100 50 10 25

Storage conditions: 10 mM Tris-HCl (pH 8.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Store at -20°C. Storage at -70 °C is recommended for periods longer than 3 months
Ligations:
After 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated. Of these 80% can be recut
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C. Reagents Supplied with Enzyme 10 X SE-buffer O
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CG methylation.
Notes:
References:
Kileva, E.V., Dedkov, V.S., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1996).

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