Bmt I

Description

Recognition site and hydrolysis position:

Source: An E.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (Hind III-digest)
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13.BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.
References:
Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K. Biotekhnologiya 1: 11-15 (2003).