Bsp1720 I

6,500.00 ₽ – 26,000.00 ₽

  • CNY: 575.44 ¥ - 2,301.78 ¥

Restriction endonuclease Bsp1720 I

  • Recognition site: GC↑TNAGC / CGANT↓CG
  • Источник: Bacillus species 1720
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E185 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E185S500 uRegular10 000 u/ml6,500.00 ₽
  • CNY: 575.44 ¥
E186L2500 uRegular10 000 u/ml26,000.00 ₽
  • CNY: 2,301.78 ¥

Description

Recognition site and hydrolysis position:

GCTNAGC GC↑TNAGC
CGANTCG CGANT↓CG

Source: Bacillus species 1720
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
50 100 50 50 75 75

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme about 80% of DNA fragment can be ligated. Of these about 95% can be recut
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.T., Tepavicharova, I.I., Mechandjiska, L.I., Builieva, E.I. Nucleic Acids Res. 19: 2504 (1991).

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