BssT1 I

BssT1 I

Price range: 2 500 ₽ through 10 000 ₽

Restriction endonuclease BssT1 I

  • Recognition site:
    5'… C▼CWWGG …3'
    3'… GGWWC▲C …5'
  • Source: Bacillus stearothermophilus T1
  • Optimal buffer: SE-буфер 2K
  • Optimal temperature: 60
  • Inactivated during 20 minutes at: 80
SKU: SE-E207 Category:

SKUРackagePackageVariantConcentrationPrice 
E207S1000 uRegular10 000-20 000 u/ml2 500 ₽
E208L5000 uRegular10 000-20 000 u/ml10 000 ₽

Description

Recognition site and hydrolysis position:

CCWWGG C↑CWWGG
GGWWCC GGWWC↓C

Source: Bacillus stearothermophilus T1
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer 2K
Enzyme activity (%):

B G O W Y ROSE
10 25 25 75 10 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 60°C. Reagents Supplied with Enzyme 10 X SE-buffer 2K
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity
References:
Serov, G.D., Tereshenko, T.A., Puchkova, L.I., Rechkunova N.I., Degtyarev, S.K., Unpublished observations.

Additional Information

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