BssT1 I

1,950.00 ₽ – 7,800.00 ₽

  • CNY: 151.65 ¥ - 606.61 ¥

Restriction endonuclease BssT1 I

  • Recognition site: C↑CWWGG / GGWWC↓C
  • Источник: Bacillus stearothermophilus T1
  • Оптимальный буфер: SE-буфер 2K
  • Оптимальная температура: 60
  • Температура инактивации, 20 мин при: 80
SKU: SE-E207 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E207S1000 uRegular10 000-20 000 u/ml1,950.00 ₽
  • CNY: 151.65 ¥
E208L5000 uRegular10 000-20 000 u/ml7,800.00 ₽
  • CNY: 606.61 ¥

Description

Recognition site and hydrolysis position:

CCWWGG C↑CWWGG
GGWWCC GGWWC↓C

Source: Bacillus stearothermophilus T1
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer 2K
Enzyme activity (%):

B G O W Y ROSE
10 25 25 75 10 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 60°C. Reagents Supplied with Enzyme 10 X SE-buffer 2K
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity
References:
Serov, G.D., Tereshenko, T.A., Puchkova, L.I., Rechkunova N.I., Degtyarev, S.K., Unpublished observations.

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