BstDS I

5,750.00 ₽ – 23,000.00 ₽

  • CNY: 467.59 ¥ - 1,870.36 ¥

Restriction endonuclease BstDS I

  • Recognition site: C↑CRYGG / GGYRC↓C
  • Источник: Bacillus stearothermophilus DS
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 65
  • Температура инактивации, 20 мин при: 80
SKU: SE-E083 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E083S1000 uRegular10 000 u/ml5,750.00 ₽
  • CNY: 467.59 ¥
E084L5000 uRegular10 000 u/ml23,000.00 ₽
  • CNY: 1,870.36 ¥

Description

Recognition site and hydrolysis position:

CCRYGG C↑CRYGG
GGYRCC GGYRC↓C

Source: Bacillus stearothermophilus DS
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
0 75 50 25 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).

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