Ege I

2,300.00 ₽ – 9,200.00 ₽

  • CNY: 187.04 ¥ - 748.14 ¥

Restriction endonuclease Ege I

  • Recognition site: GGC↑GCC / CCG↓CGG
  • Источник: Enterobacter gergoviae
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E243 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E243S200 uRegular5 000 u/ml2,300.00 ₽
  • CNY: 187.04 ¥
E244L1000 uRegular5 000 u/ml9,200.00 ₽
  • CNY: 748.14 ¥

Description

Recognition site and hydrolysis position:

GGCGCC GGC↑GCC
CCGCGG CCG↓CGG

Source: Enterobacter gergoviae
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (HindIII-digest)
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 75 10 50 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycero; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Dedkov, V.S., Myakisheva, T.V., Kileva, E.V., Degtyarev, S.K. Unpublished observations (1996).

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