PspE I

PspE I

Price range: 2 900 ₽ through 11 600 ₽

Restriction endonuclease PspE I

  • Recognition site:
    5'… G▼GTNACC …3'
    3'… CCANTG▲G …5'
  • Source: Pseudomonas species E
  • Optimal buffer: SE-buffer B
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E169 Category:

SKUРackagePackageVariantConcentrationPrice 
E169S1000 uRegular10 000-20 000 u/ml2 900 ₽
E170L5000 uRegular10 000-20 000 u/ml11 600 ₽

Description

Recognition site and hydrolysis position:

GGTNACC G↑GTNACC
CCANTGG CCANTG↓G

Source: Pseudomonas species E
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 50 25 50 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
References:
Dedkov, V.S., Rechkunova, N.I., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1994).

Additional Information

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