PspE I

2,300.00 ₽ – 9,200.00 ₽

  • CNY: 187.04 ¥ - 748.14 ¥

Restriction endonuclease PspE I

  • Recognition site: G↑GTNACC / CCANTG↓G
  • Источник: Pseudomonas species E
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E169 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E169S1000 uRegular10 000-20 000 u/ml2,300.00 ₽
  • CNY: 187.04 ¥
E170L5000 uRegular10 000-20 000 u/ml9,200.00 ₽
  • CNY: 748.14 ¥

Description

Recognition site and hydrolysis position:

GGTNACC G↑GTNACC
CCANTGG CCANTG↓G

Source: Pseudomonas species E
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 50 25 50 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
References:
Dedkov, V.S., Rechkunova, N.I., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1994).

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