Description
Recognition site and hydrolysis position:
| GCATC(N)5 | GCATC(N)5↑ |
| CGTAG(N)9 | CGTAG(N)9↓ |
Source: An E.coli strain that carries the cloned SfaN I gene from Streptococcus faecalis N
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 10 | 25 | 100 | 75 | 0 | 25 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.Reagents Supplied with Enzyme
10 X SE-buffer O
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Schildkraut, I., Greenough, L. Unpublished observations.
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006
