Tth111 I

2,300.00 ₽ – 9,200.00 ₽

  • CNY: 187.04 ¥ - 748.14 ¥

Restriction endonuclease Tth111 I

  • Recognition site: GACN↑NNGTC / CTGNN↓NCAG
  • Источник: Из штамма E.coli несущего клонированный ген Tth111 I из Thermus Thermophilus 111
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 65
  • Температура инактивации, 20 мин при: 80
SKU: SE-E097 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E097S400 uRegular5 000 u/ml2,300.00 ₽
  • CNY: 187.04 ¥
E098L2000 uRegular5 000 u/ml9,200.00 ₽
  • CNY: 748.14 ¥

Description

Recognition site and hydrolysis position:

GACNNNGTC GACN↑NNGTC
CTGNNNCAG CTGNN↓NCAG

Source: An E.coli strain, that carries the cloned gene Tth111I from Thermus Thermophilus 111
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (HindIII-digest)
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 50 10 10 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 500 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme 10% of the DNA fragments can be ligated.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentrationmay result in star activity.
References:
Shinomiya, T., Sato, S. Nucleic Acids Res. 8: 43-56 (1980).

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