We have discovered a bacterial strain, Peribacillus species 31, which is a producer of a new restriction endonuclease Pse31I.
The enzyme recognizes a six-nucleotide non-palindromic DNA sequence, 5’-GGTCTC-3’, and cleaves it away from the recognition site at positions
1/5: 5’-GGTCTC(N)1^ / 3’-CCAGAG(N)5^-5’. Thus, Pse31I is a true isoschizomer of the restriction enzyme Eco31I.
The producer strain was identified based on morphological and biochemical characteristics, as well as the primary structure analysis of a 16S rRNA gene fragment.
Pse31I exhibits maximum activity at 37°C and is inactivated by incubation at 55°C and 65°C.
The enzyme does not cleave sites with methylated cytosine in the top strand (5’-GGTCT(5mC)-3’) and/or the bottom strand (3’-(5mC)CAGAG).
The optimal working conditions for the enzyme are SE buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37°C.
The enzyme is available in both regular version and the Turbo version for rapid hydrolysis.
You can order the enzyme on our website.