Acs I

Enzyme name Acs I
Prototype ApoI
Known analogs ApoI, XapI
SKU SE-E013
Turbo version Available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… R▼AATTY …3'
3'… YTTAA▲R …5'
Source Arthrobacter citreus
Optimal buffer SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature 50 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
25505010010100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer W, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  2. S. Kh. Degtyarev, A.A. Kolyhalov, N.I. Rechkunova, M.A. Abdurashitov Acs I, a new restriction endonuclease from Arthrobacter citreus 310 recognizing 5′-Pu^AATTPy-3′. // Nucleic Acids Research, Vol. 20, No.14 (1992).
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