Acs I

Enzyme nameAcs I
PrototypeApoI
Known analogsApoI, XapI
SKUSE-E013
Turbo versionAvailable
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… R▼AATTY …3'
3'… YTTAA▲R …5'
SourceArthrobacter citreus
Optimal bufferSE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature50 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
25505010010100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer W, BSA
NotesTo obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  2. S. Kh. Degtyarev, A.A. Kolyhalov, N.I. Rechkunova, M.A. Abdurashitov Acs I, a new restriction endonuclease from Arthrobacter citreus 310 recognizing 5′-Pu^AATTPy-3′. // Nucleic Acids Research, Vol. 20, No.14 (1992).
// Код для некролога