AsuC2 I

Enzyme nameAsuC2 I
PrototypeCauII
Known analogsBcnI, CauII, NciI
SKUSE-E257
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CC▼SGG …3'
3'… GGS▲CC …5'
SourceActinobacillus suis CA
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature65 °C
Buffer activity
BGOWYROSE
7550102510040
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion less than 10% of the DNA fragments can be ligated
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
References
  1. Unpublished data Kileva, E.V., Dedkov, V.S., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.Kh Unpublished observations (1996).
// Код для некролога