AsuC2 I

Enzyme name AsuC2 I
Prototype CauII
Known analogs BcnI, CauII, NciI
SKU SE-E257
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CC▼SGG …3'
3'… GGS▲CC …5'
Source Actinobacillus suis CA
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7550102510040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion less than 10% of the DNA fragments can be ligated
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Unpublished data Kileva, E.V., Dedkov, V.S., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.Kh Unpublished observations (1996).
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