Bsc4 I

Enzyme nameBsc4 I
PrototypeBsiYI
Known analogsBseLI, BsiYI, BslI
SKUSE-E219
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CCNNNNN▼NNGG …3'
3'… GGNN▲NNNNNCC …5'
SourceBacillus schlegelii 4
Optimal bufferSE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature55 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
7575501002590
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer W, BSA
NotesTo obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. V.S. Dedkov Defining the Specificity of DNA Methyltransferase M.Bsc4I in Cellular Lysate by Blocking Restriction Endonucleases and Computer Modeling // Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya (Moscow), No 3, p.114-120 (2009).
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