Unpublished data
Unpublished observations.
Bsp13 I
| Enzyme name | Bsp13 I | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Prototype | BspMII | ||||||||||||
| Known analogs | AccIII, Aor13HI, BlfI, BseAI, BspEI, BspMII, Kpn2I, MroI | ||||||||||||
| SKU | SE-E183 | ||||||||||||
| Turbo version | Not available | ||||||||||||
| High-concentration version | Not available | ||||||||||||
| Restriction type | Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites. | ||||||||||||
| Recognition site | 5'… T▼CCGGA …3' 3'… AGGCC▲T …5' | ||||||||||||
| Source | Bacillus species 13 | ||||||||||||
| Optimal buffer | SE-buffer 2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) | ||||||||||||
| Optimal temperature | 50 °C | ||||||||||||
| Inactivation temperature | 65 °C | ||||||||||||
| Buffer activity |
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| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 50°C in a total reaction volume of 50 μl. | ||||||||||||
| Assayed on | Lambda DNA (dam-) | ||||||||||||
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. | ||||||||||||
| Ligation | After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. | ||||||||||||
| Nonspecific hydrolysis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C. | ||||||||||||
| Methylation sensitivity | Blocked by overlapping Dam methylation (G m ATC) TCCG GA TC and GA TC CGGA. | ||||||||||||
| Reagents supplied with enzyme | 10 X SE-buffer 2K | ||||||||||||
| References |